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Rat brain dissection protocol

rat brain dissection protocol

Ventral - the belly or lower surface.
Striatum (caudate nucleus) dissection Step 6 To collect the hippocampus, put the ventral side of the brain up and then remove midbrain to expose the hippocampus.
Made ice cold before use.
Collect the frontal cortex right after the second cut, using a blade and a Dumont.
Anti-MAP-2 was a rabbit polyclonal from Cell Signaling (Danvers, MA anti-gfap antibody was a mouse monoclonal from Chemicon (Millipore, Billerica, MA and the mouse monoclonal anti-GRB2 antibody was from BD Transduction Laboratories (Sparks, MD).Within the cells, these molecules may be burned to release energy for cellular activity, built into the structural elements of the cell, or stored world war z ios game for later use.You should now be able to open the flaps of skin.Orientation terms as depicted on a rat (lateral view).Dapi staining, in blue, indicates cellular nuclei.

This decreases the probability of dislocation and injury when the bird is landing.
Hind limb - The thigh bone is the 'femur'.
Distal - farther from the midline of the body.
Step 3, collect the olfactory bulb right after the first cut.
Summary, micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases.Hippocampus dissection Discussion Different brain regions associate with different neurodegenerative diseases.Diaphragm - Notice that the lungs are located within closed cavities, the thoracic or pleural cavities, which are lined by membranes, called the pleura.Now compare the trunk regions.Stomach - The food passes from the esophagus into the stomach.Affiliation(s (1) Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, VU University, Amsterdam, The Netherlands.The cutting positions from the medial view of rat hemisphere.In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol.It connects to the scapula with a ball and socket type of joint.The primary function of the large intestine is to absorb most of the water of the digestive secretions, conserving it for use within the body.